细胞因子
Catalog Number: KD-002
Pr…
Species
Human
Protein Construction
IL-3 (Ala20-Phe152), Accession # AAC08706
Tag
No tag |
Expression System
HEK 293
Purity
> 95% as determined by SDS-PAGE
Predicted Molecular Mass
15 kDa SDS-PAGE17-30 kDa, under reducing conditions |
Endotoxin
<0.1 EU/μg of the protein by the LAL method.
Biological Activity
Measured in a cell proliferation assay using TF-1 human erythroleukemic cells. The ED50 for this effect is 0.15-0.75 ng/mL.Formulation
| Lyophilized from a 0.22μm filtered solution in PBS |
Reconstitution
| It is recommended that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute the lyophilized powder at 100 μg/mL in sterile deionized water |
Storage&Stability
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, at lower than -70 °C as supplied.
1 week, 2 to 8 °C under sterile conditions after reconstitution.
1 month, -20 to -70 °C under sterile conditions after reconstitution.
Usage
Research use only |
Background
| Interleukin-3 is a pleiotropic factor produced primarily by activated T cells that can stimulate the proliferation and differentiation of pluripotent hematopoietic stem cells as well as various lineage committed progenitors. In addition, IL-3 also affects the functional activity of mature mast cells, basophils, eosinophils and macrophages. In addition to activated T cells, other cell types such as human thymic epithelial cells, activated murine mast cells, murine keratinocytes and neurons/ astrocytes can also produce IL-3.IL-3 exerts its biological activities through binding to specific cell surface receptors. The high affinity receptor responsible for IL-3 signaling is composed of at least two subunits, an IL-3 specific α chain which binds IL-3 with low affinity and a common β chain that is shared by the IL-5 and GM-CSF high-affinity receptors. Although the β chain itself does not bind IL-3, it confers high-affinity IL-3 binding in the presence of the α chain. Receptors for IL-3 are present on bone marrow progenitors, macrophages, mast cells, eosinophils, megakaryocytes, basophils and various myeloid leukemic cells. |
Data
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