Human        

FGF-2 (Ala135-Ser288), Accession # P09038-4       

Tag    

E. coli        

<0.1 EU/μg of the protein by the LAL method.         

Measured in a cell proliferation assay using NIH-3T3 mouse embryonic fibroblasts. The ED50 for this effect is 0.13-1 ng/mL.          

Lyophilized from a 0.22μm filtered solution in PBS  

It is recommended that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute the lyophilized powder at 100 μg/mL in sterile deionized water   

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.         

12 months from date of receipt, at lower than -70 °C as supplied.

1 week, 2 to 8 °C under sterile conditions after reconstitution.

1 month, -20 to -70 °C under sterile conditions after reconstitution.

Background    

FGF-2 (fibroblast growth factor-2), also commonly known as bFGF (basic Fibroblast Growth Factor), is a member of the FGF family. It binds FGFRs and heparan sulfate and modulate the function of a wide range of cell types. Once the FGF2/FGFR/HSPG complex is formed, it triggers a cascade of signals inside the cell (primarily the MAPK/ERK and PI3K/AKT pathways), which ultimately lead to changes in gene expression and the cellular responses. FGF-2 is a pleiotropic molecule, meaning it has multiple, diverse effects depending on the context and type of cell it acts upon. Its main functions include: cell proliferation, angiogenesis, cell survival (anti-apoptosis), tissue repair and wound healing, embryonic Development and nervous system function.