细胞因子
Catalog Number: KD-057
Species
Human
Protein Construction
IL-4 (His25-Ser153), Accession # P05112-1
Tag
C-terminal 8-His tag |
Expression System
HEK 293
Purity
> 95% as determined by SDS-PAGE
Predicted Molecular Mass
15 kDa SDS-PAGE18.4 kDa, under reducing conditions |
Endotoxin
<0.1 EU/μg of the protein by the LAL method.
Biological Activity
Measured in a cell proliferation assay using TF-1 human erythroleukemic cells. The ED50 for this effect is 0.05-0.35 ng/mL.
Formulation
Lyophilized from a 0.22μm filtered solution in PBS
Reconstitution
It is recommended that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute the lyophilized powder at 100 μg/mL in sterile deionized water
Storage&Stability
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, at lower than -70 °C as supplied.
1 week, 2 to 8 °C under sterile conditions after reconstitution.
1 month, -20 to -70 °C under sterile conditions after reconstitution.
Usage
Research use only |
Background
Interleukin-4 (IL-4) is a pleiotropic cytokine regulates diverse T and B cell responses including cell proliferation, survival, and gene expression. It has important effects on the growth and differentiation of different immunologically competent cells. Interleukin-4 is produced by mast cells, T cells, and bone marrow stromal cells. IL-4 regulates the differentiation of native CD4+ T cells (Th0 cells) into helper Th2 cells, and regulates the immunoglobulin class switching to the IgG1 and IgE isotypes. IL-4 has numerous important biological functions including stimulating B-cells activation, T-cell proliferation and CD4+ T-cells differentiation to Th2 cells. It is a key regulator in hormone control and adaptive immunity. IL-4 also plays a major role in inflammation response and wound repair via activation of macrophage into M2 cells.
Data
返回产品列表 
查看更多详情