细胞因子
Catalog Number: KD-009
Pr…
Species
Human
Protein Construction
IL-1ꞵ (Ala117-Ser269), Accession # NP_000567
Tag
No tag |
Expression System
E. coli
Purity
> 95% as determined by SDS-PAGE
Predicted Molecular Mass
17 kDa SDS-PAGE17 kDa, under reducing conditions |
Endotoxin
<0.1 EU/μg of the protein by the LAL method.
Biological Activity
Measured by its ability to induce IFN-γ secretion by NK-92 human natural killer lymphoma cells. The ED50 for this effect is <5 ng/mL.
Formulation
Lyophilized from a 0.22μm filtered solution in PBS
Reconstitution
It is recommended that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute the lyophilized powder at 100 μg/mL in sterile deionized water
Storage&Stability
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, at lower than -70 °C as supplied.
1 week, 2 to 8 °C under sterile conditions after reconstitution.
1 month, -20 to -70 °C under sterile conditions after reconstitution.
Usage
Research use only |
Background
Interleukin-1 beta (IL-1ꞵ) is a proinflammatory cytokine produced in a variety of cells including monocytes, tissue macrophages, keratinocytes and other epithelial cells. Both IL-1 alpha and IL-1 beta binds to the same receptor and has similar if not identical biological properties. These cytokines have a broad range of activities including, stimulation of thymocyte proliferation, by inducing IL-2 release, B-cell maturation and proliferation, mitogenic FGF-like activity and the ability to stimulate the release of prostaglandin and collagenase from synovial cells. However, whereas IL-1 beta is a secreted cytokine, IL-1 alpha is predominantly a cell-associated cytokine.
Data
返回产品列表 
查看更多详情