细胞因子
Catalog Number: KD-013
Pr…
Species
Human
Protein Construction
IL-18 (Tyr37-Asp193), Accession # Q14116
Tag
No tag |
Expression System
E. coli
Purity
> 95% as determined by SDS-PAGE
Predicted Molecular Mass
18 kDa SDS-PAGE17 kDa, under reducing conditions |
Endotoxin
<0.1 EU/μg of the protein by the LAL method.
Biological Activity
Measured by its ability to induce IFN-γ secretion by KG‑1 cells. The ED50 for this effect is <20 ng/mL.
Formulation
Lyophilized from a 0.22μm filtered solution in PBS with 5% Trehalose
Reconstitution
It is recommended that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute the lyophilized powder at 100 μg/mL in sterile deionized water
Storage&Stability
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, at lower than -70 °C as supplied.
1 week, 2 to 8 °C under sterile conditions after reconstitution.
1 month, -20 to -70 °C under sterile conditions after reconstitution.
Usage
Research use only |
Background
Interleukin-18 (IL18, also known as interferon-gamma inducing factor or IGIF) is a cytokine that belongs to the IL-1 superfamily and is produced by macrophages and other cells. Its biological activity is pleiotropic and it has been shown to induce interferon-gamma production in KG-1 cells. The combination of this cytokine and IL12 has been shown to inhibit IL4 dependent IgE and IgG1 production, and enhance IgG2a production in B cells.
Data
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